KMID : 1134819970260040620
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Journal of the Korean Society of Food Science and Nutrition 1997 Volume.26 No. 4 p.620 ~ p.624
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Optimum Condition for Pigment Production and Antioxidative Activity of the Products by Bacillus subtilis DC - 2 with Response Surface Methodology
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Choi Ung-Kyu
Ji Won-Dae Chung Hyun-Chae Choi Dong-Hwan Chung Yung-Gun
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Abstract
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The conditions for color intensity and electron donating ability to ¥á,¥á-diphenyl-¥â-picryl-hydrazyl (DPPH) of Bacillus subtilis DC-2 were investigated. Temperature, pH and cultivation time were chosen as three factors, and the optimal conditions of color intensity and DPPH was determined with response surface methodology. Color intensity was affected by cultivation temperature(p£¼0.1). DPPH was influenced by cultivation temperature(p£¼0.05) and pH(p£¼0.1). But cultivation time was affected neither color intensity nor DPPH. Optimal conditions of color intensity with Bacillus subtilis DC-2 were appeared at cultivation temperature of 39.25¡É, pH 8.83 and cultivation time of 84.41hrs. Optimal conditions of DPPH with Bacillus subtilis DC-2 were revealed at cultivation temperature of 39.19¡É, pH 8.84 and cultivation time of 82.21hrs.
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KEYWORD
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Bacillus subtilis DC-2, pigment generation, electron donating ability, DPPH, response surface methodology
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